Construction of DNA l ibrary of Spirulina and its appl ication in cloning genes
钝顶螺旋藻基因组质粒文库的构建及其在获得功能基因上的应用

The genomic DNA isolated from Spirulina was digested by Sau3AI.The DNA fragments with length of 1~2 kb were cloned into plasmid vector pBR322 which was previously digested with BamHI and dephosphorylated with calf alkaline phosphatase.The recombinant plasmid was transformed into Escherichia coli Top10 competent cells and the genomic library was constructed.Using the anchor primer and degenerated primer,we obtain 5'end of STK in Spirulina from this genomic library.