Preparation of hemocyanin polyclonal antibody and the identification of 35 kDa hemocyanin fragment in Haliotis diversicolor Reeve
杂色鲍血蓝蛋白多克隆抗体的制备与血蓝蛋白35kDa片段鉴定

Haliotis diversicolor Reeve, was taken as research material. High purity hemocyanin proteins were purified from the hemolymph of abalone using CsCl density gradient centrifugation method. By injection of the purified antigen to a rabbit, high titer polyclonal anti-serum was acquired. The anti-serum was further processed with the protein A affinity purification to produce purified antibodies. Finally, the purified polyclonal antibodies with higher titer and specificity were acquired. Further Western blotting with these antibodies, plenty of hemocyanin was found as derivates of various molecular weights in abalone hemolymph.