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海洋科学 2008
Islation of a PAHs degrading marine bacterium Nah-1 and its degradative gene
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Abstract:
A PAHs-degrading psychrophilic bacterium,with naphthalene as sole carbon and energy source, was isolated from deep-sea sediment of Southern Okinawa Trough.The growth curve of this bacterium was determined and the optimum growth conditions of this bacterium were studied.16S rDNA sequences analy- sis indicated that it belongs to the genus Cycloclasticus.A fragment of the degrading dioxygenase gene was obtained through PCR from Nab-1.And sequence alignment revealed that it was similar to the phnA1 gene of Cycl...