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海洋科学 2011
RAPD analysis of genetic structure and differentiation of Coelomactra antiquata
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Abstract:
The Randomly Amplified Polymorphic DNA(RAPD) technique was applied to assess the genetic variations among five stocks Changle(CL),Qidong(QD),Lianyungang(LYG),Shandong(JN) and Guangxi(BH)] of Coelomactra antiquata using 55 random primers.Genetic diversity was analyzed with PopGen(Version 1.31) software.A total of 12 highly reproducible primers and 88 clear and stable bands were obtained.The lengths of amplified fragments were from 100 bp to 3000 bp.The primer S299,which amplified 600 bp fragment,was specifi...