Characterization of promoters from flounder (Paralichthys olivaceus) muscle-regulatory genes, MyoD and Myf5
用显微注射方法分析牙鲆肌肉发育调节基因MyoD,Myf5的启动子序列

The promoter sequences of flounder muscle regulatory gene,MyoD and Myf5,are cloned as 608 bp and 1 073 bp respectively.Many muscle regulatory gene binding sites are found in the promoters of MyoD and Myf5.The promoter sequences were ligated to GFP vector and constructed as MyoDP-GFP and Myf5P-GFP.The result constructs were microinjected into one-cell or two-cell stage zebrafish embryos to make a transient expression analysis.The results showed that the 608 bp MyoD promoter and the 1 073 bp Myf5 promoter can drive GFP specifically expressed in muscle fibers in zebrafish.It can be seen that the two promoters contain muscle specific regulatory elements,and they can function across species.