Effects of nitrate and phosphate on the biochemical composi-tion of Thalassiosira weissflogii
氮磷营养盐对微氏海链藻细胞生化组成的影响

Thalassiosira weissflogii was cultured in high levels of nutrients, and the influence of different levels of two macro-nutrients, nitrate and phosphate on biochemical composition, including the contents of carbohydrate, protein, chlorophyll, basic function group, cell dry weight and cell shape was investigated to assess the bioactivity, surface adsorption capability, i.e., the metal uptake/adsorption ability by T. weissflogii. When the ratio of nitrate to phosphate n(N):n(P) is 16, the contents of chlorophyll, carbohydrate, and protein were the highest, its growth level and bioactivity were the best, and its ability to absorb the trace element were also outstanding. But the quantity of basic function group in cellular wall and cell size was the highest when n(N):n(P) is 8, the lowest when n(N):n(P) is 64. So, the ability for bio-absorption trace element is good when n(N):n(P) is 8, and poor when n(N):n(P) is 64. So, the influences of n(N):n(P) on the metal uptake and adsorption were quite different.