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海洋科学  2011 

The analysis of the low coverage Porphyra yezoensis draft genome
条斑紫菜低覆盖度基因组草图分析

Keywords: Porphyra yezoensis,Solexa high-throughput sequencing system,metabolic pathway
条斑紫菜(Porphyra
,),Solexa,高通量测序,代谢通路

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Abstract:

We constructed the low-coverage Porphyra yezoensis draft genome using the Solexa high-throughput sequencing system. The predicted genome size was approximately 220 Mbp, with the GC content of 53.09%. The draft genome contained 26 629 predicted protein-coding genes, 16 409 of which had introns, averagely 2.22 introns per gene. Gene structure analysis of intron-containing genes showed that the average gene size and intron size was 2214 bp and 319 bp, respectively. Metabolic pathway analysis indicated that the purine metabolism was the largest pathway. The P. yezoensis draft genome constructed in this study manifested important genomic properties such as genome size and the gene structure of protein-coding gene, proving the feasibility of Solexa high-throughput system in sequencing the whole genome of P. yezoensis.

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