RAPD-PCR study on the genetic diversity of sponge-associated microorganisms
海绵共附生微生物基因多态性的RAPD-PCR分析

Sponge-associated microorganisms were isolated from 4 species of sponges Stelletta tenui (Lindgren, 1897), Halichondria rugosa (Ridley & Dendy), Craniella australiensis (Carter), Dysidea avara (Schmidt) in the South China Sea. After cultivation the genetic diversity of 64 strains of sponge-associated Bacillus (16 strains /sponge) was revealed based on RAPD-PCR fingerprinting and clustering analysis. It was proved that we can obtain sponge-associated microorganisms by traditional isolation method and there is genetic diversity among strains from the same or different sponges.