Genetic diversity of different geographical stocks of Epinephelus coioides by microsatellite DNA
斜带石斑鱼不同地理群体遗传变异的微卫星分析

The microsatellite DNA was used to evaluate genetic diversity and genetic differentiation in two geographical stocks of Epinephelus coioides from Taiwan and Guangdong. Altogether 5 primers were assessed in 46 individuals in each stock by analyzing the heterozygosity, Hardy-Weinberg balace deflection index, genetic distance, genetic differentiation. The result showed that the average number of alleles in each microsatellite locus was 4.2, the average observed heterozygosity (Ho) of two stocks was 0.672 1 and 0.682 6, the average expected heterozygosity (He) was 0.647 9 and 0.544 4 respectively, and genetic differentiation (FST) was 0.011 0. It seemed that these two geographical stocks of E. coioides still kept high genetic diversity, and the genetic differentiation among stocks was close to the average level of marine fish.