Proteomic analysis of aerobic degradation of highly chlorinated PCBs by Pseudomonas putida HP1
恶臭假单胞菌好氧降解高氯联苯的蛋白质组分析

The total proteins of Pseudomonas putida HP1 that use glucose and Arcolor 1260 as nutritional matrix for growth were separated respectively by immobilized pH gradient-based 2-DE. The differential expressed proteins were screened by PD Quest analysis software and identified by peptide mass fingerprint based on MOLDI-TOF-MS. Part of differential protein spots were identified in reference of the database. These results showed that a well-resolved and reproducible 2-DE pattern was obtained,and eighty differential expressed protein spots were found by comparative analysis. Fourteen differential expressed proteins were identified, including stress response proteins, proteins related to protein biosynthesis, transporter and metabolic enzymes. This suggested that the cell using PCBs as sole carbon and energy source produced stress response, and then maintained their stability, growth and metabolism by enhancing the expression of stress response proteins, proteins related to protein biosynthesis and enzymes related to metabolism.