Study on biodiversity in two-phase integrated sludge thickening and digestion (TISTD) reactor
两相一体式污泥浓缩消化(TISTD)反应器微生物多样性研究

16S rDNA and PCR-TGGE molecular biology methods are applied to investigate biodiversity and microbial community structure in the TISTD reactor. The biodiversity and microbial community structure of different chambers of the reactor at different stages are obtained. The results show microbial community structure in the reactor present great biodiversity. The dominating microbial community is obvious and the microbial community structure varies greatly. The outer chamber has a greater microbial community population than the inner chamber, revealing that the outer chamber is the main place for hydrolytic acidification while the strictly anaerobic inner chamber is the main methanogenic place. Furthermore, different dominant population can be found in different reactor operating periods. With the loading changes, microbial community structure and population show a clear succession and the dominant microbial community is in dynamic change. Under the effect of synergistic and competition growth, a specific microbial community structure has been formed. The results further explain the working mechanism of thickening and digestion which provides a theoretical basis for the popularization, application and optimization of the TISTD reactor.