The effect of oral exposure to Perfluorooctane Sulfonate(PFOS) on spleen T cell subsets CD4+/CD8+ of C57BL/6 mice
全氟辛烷磺酸(PFOS)经口染毒对C57BL/6小鼠脾脏T细胞亚群CD4+/CD8+的影响

The objective of this experiment was to assess the effects of Perfluorooctane Sulfonate(PFOS) on spleen Tcell subsets through detecting the average positive rate and the numbers of splenic subpopulations of CD4+ and CD8+ Tcells.Twenty-four adult C57BL/6 male mice were divided into four groups.Animals were given0,5,10 and 20 mg·kg-1 PFOSby oral gavage every day for 12 days,and then single-cell suspensions of splenocytes were prepared.After labeling with the Fluorescein Isothiocyanate(FITC) conjugated rat-anti-mouse CD3(monoclonal),phycoerythrin(PE) conjugated rat anti-mouse CD4(monoclonal) and peridinin chlorophyl protein(Percp) conjugated rat anti-mouse CD8(monoclonal),the phenotypes of splenocyte populations were analyzed by Flow Cytometry(FCM).The results showed that in the 12-d study,body weight of mice exposed to 10 mg·kg-1 and 20 mg·kg-1 of PFOSshowed significant deterioration from their own pre-exposed baseline.Spleen and thymus mass were significantly decreased compared to the control following exposure to 20 mg·kg-1 of PFOS.Furthermore,liver mass was increased in each exposure group.Flow cytometric analysis showed that the average positive rate and the numbers of splenic subpopulations of CD4+ and CD8+ cells decreased significantly in the 20 mg·kg-1 PFOSgroup in comparison with normal C57BL/6 mice.This study indicates that PFOSmay decrease the average positive rate of CD4+ and CD8+T-cell in spleen organs,thus suppressing immune function.