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环境科学学报 2009
Roles of cortisol exposure on functional genes in the HPA axis in male Chinese rare minnow (Grobiocypris rarus)
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Abstract:
The effects of cortisol exposure on functional genes in the HPA axis in male Chinese rare minnow(Grobiocypris rarus)were studied.Segments of CRF,POMC and GR of the Chinese rare minnow were cloned and quantitative PCR methods were used to determine the effects on expression patterns of CRF and POMC in the brain and GR in the liver of male Chinese rare minnows treated with cortisol.BLAST analysis revealed high levels of similarity at the amino acid level(>90%)to corresponding genes of previously published seq...