Labeling gram-negative bacteria using green fluorescent protein
利用绿色荧光蛋白标记革兰氏阴性细菌的研究

In this study, four transposable plasmids were constructed, pTnMod-OCm-G, pTnMod-OTc-G, pTnMod-OKm3-G and pTnMod-OGm-G, which can constitutively express green fluorescent protein. Via triparental mating, GFP gene was inserted into genomes of Sphingomonas sp. 12A and Pseudomonas sp. 12B, two polycyclic aromatic hydrocarbons-degrading bacterial strains, respectively. The transformants which can degrade polycyclic aromatic hydrocarbons, can constitutively express GFP without the presence of antibiotics through many generations of propagation. The transposons can be transferred to other gram negative bacteria and expressed stably. Therefore, they have the potential to be used in research on bacterial survival and ecological safety in contaminated environments.