Heterologous expression of a synthetic gene encoding lignin peroxidase H2 in Pichia pastoris
木质素过氧化物酶LiPH2合成基因在毕赤酵母中的表达

After optimizing the gene sequence,a lignin peroxidase H2(LiPH2) gene was chemically synthesized and then successfully inserted into selected expression vectors. The recombinant vectors containing different secretion signals and selectable markers were then transformed into the Pichia pastoris genome. DOA extracted from the recombinant P. pastoris was amplified using the specific primers and analyzed by electrophoresis to confirm the correct integration of the LiPH2 gene in the DNA sequence. Eight different...