Online biomonitoring for waterborne antibiotics and organophosphorous using the Multispecies Freshwater Biomonitor
利用多物种净水监测仪在线监测水体抗生素药物及有机磷农药

The movement behavior of Danio rerio was studied as a bioindicator of contamination by antibiotics (Enrofloxacin, Sulfadiazine) and organophosphorous pesticides (Dimethoate, Malathion) using an online biomonitoring method, the Multispecies Freshwater Biomonitor (MFB). A static test of acute toxicity revealed the 96 h LC50 values (95% confidence limit) for Danio rerio to be 61 mg·L-1 for Dimethoate and 5.4 mg·L-1 for Malathion. No acute toxicity for Enrofloxacin and Sulfadiazine to Danio rerio was observed during the 96 h exposure period. The behavioral response of Danio rerio was sensitive to exposure to antibiotics and organophosphorous pesticides. Increasing concentration resulted in more intensive behavioral responses of Danio rerio with more short response time, which could be described by the Stepwise Stress Model (SSM). The behavior change of Danio rerio exhibited a positive relation to the acute toxicity test. Therefore, movement behavior can be effectively applied as a bioindicator and early warning of antibiotic and organophosphorous pesticide contamination by online biomonitoring.