Isolation and characterization of protease-producing Bacillus sp. TC16 and its enzyme features
污泥中一株产耐高温蛋白酶菌株的分离、鉴定及酶学特性

Proteases are a kind of enzymes that can hydrolyze protein peptide bond and decompose proteins into amino acids which could then be absorbed and utilized directly by animals and plants. The method of clear zone in combination with protease activity detection was used to test the protease activity of a screened sewage sludge-borne strain TC16. The isolate was classified into Bacillus according to its morphology, physiological and biochemical features, and 16S rDNA sequence comparison and analysis. In addition, extracellular protease activity was also detected in the cultural supernatant of the strain Bacillus sp. TC16 was identified as a mesophily bacterium with a wide range of pH and temperature tolerance as well as high enzyme activity comparing with other strains.