Characteristic and performance of phosphate accumulation by Staphyloccocus aureus
金黄色葡萄球菌聚磷特性研究

Phosphate release and uptake under anaerobic and aerobic alternative condition and carbon sources utilization of cultivated strain of Staphyloccocus aureus were investigated. Phosphate removal from domestic wastewater were also investigated. Traditionally, Staphyloccocus aureus was not recognized as a typical phosphorus accumulation organism (PAOs). The results from this study however showed that Staphyloccocus aureus cultured under normal aerobic condition exhibited noticeable phosphate release when it was shifted to anaerobic condition only for 5 hours. It would take up the phosphate quickly when transformed into the aerobic stage. The efficiency of phosphate uptake from bulk liquid was 65% of the total phosphate removal after 6 hours. As a result, the phosphate content of the strain increased from 0.98% to 2.30%. The optical photos of the strain during the culture period showed that the PHB particles content of the strain increased obviously at the anaerobic stage, while the polyphosphate particles content increased accordingly at the aerobic stage. Cultivated after 4 cycles of anaerobic and aerobic alternation, phosphate content of the strain increased from 0.8% to 4.6%, and the amount of phosphate uptake did not rise with the cultivation. Even at the first cycle, the strain displayed an outstanding ability of phosphate release and uptake. The strain could utilize various carbon sources including the macromolecule organic matters, such as glucose and peptone, for releasing phosphate at the anaerobic stage. The phosphate removal efficiency could reach 98.2% when the strain was inoculated in domestic wastewater. This demonstrates that the strain presents the typical characteristic of the PAOs, and can utilize carbon sources besides VFAs during phosphorus release. The ability of phosphate accumulation of the strain is inherent. Inducing is therefore not needed.