Inhibition effects of fullerene on the polymerase chain reaction
富勒烯对PCR反应的抑制作用

Based on a designed 110 bp single strand DNA template, a polymerase chain reaction (PCR) method was established to evaluate the effect of C60 on DNA in vitro replication. The results indicated that C60 could dose-dependently inhibit the generation of PCR products. After co-incubation with C60, the activity of treated Taq DNA polymerase was significantly inhibited, and the amplification product of the treated DNA template was also decreased. The inhibition effect induced by C60 could be eliminated by increasing the amount of Taq DNA polymerase in the PCR reaction solution, but could not be eliminated by increasing the quantity of DNA template. The results revealed that C60 could inhibit the activity of Taq DNA polymerase, and may have potential adverse effects on the DNA template.