Study on protein oxidative damage in mice by intragastric administration of nitrobenzene
硝基苯灌胃致小鼠蛋白质氧化损伤作用的研究

In order to explore the degree of protein oxidative damage in mice organs induced by nitrobenzene,different concentrations of nitrobenzene were administered by daily intragastric administration for 30 days.The protein carbonyl content was assayed with 2,4-dinitrophenylhydrazine to evaluate the degree of protein oxidative damage.No significant difference was observed between the control group and the solvent-only group(p>0.05).As the nitrobenzene concentration increased,the protein carbonyl content in liver,kidney,spleen and heart also increased,but in brain tissue,the increase was only observed in the high-dose group.In liver,the difference between the low-dose group and the control group was significant and the differences between middle or high dose groups and the solvent group were significant(p<0.05).In conclusion,low-doses of nitrobenzene caused oxidative damage to some mice tissues and damage to each tissue was greater at higher doses,but the sensitivity to nitrobenzene was different for each tissue.