Oxidative damage of mouse brain cells induced by pesticide chlorpyrifos
农药毒死蜱对小鼠脑细胞氧化损伤的研究

Chlorpyrifos is an efficient, low toxicity, broad-spectrum organophosphorus insecticide acaricide. This study aims at identifying the oxidative stress effects of chlorpyrifos on organisms. Kunming mice were orally administrated with chlorpyrifos daily for seven days at the concentration of 3, 6 and 12 mg·kg-1, respectively. The contents of reactive oxygen species (ROS), glutathione (GSH) and malondialdehyde (MDA) in the brain homogenate and DNA-protein Crosslink (DPC) coefficients in the brain cells were measured to indicate the oxidative damages. The experimental results showed that the contents of ROS, MDA and DPC coefficients increased gradually while GSH content decreased with the increasing exposure dose, and all the biomarkers were in the exposure dose-response manner. When the exposure dose was at 6 mg·kg-1, DPC coefficient was significantly higher than the control group (p<0.05), and MDA contents had extremely significant differences (p<0.01). In the higher dose groups (12 mg·kg-1), ROS content and DPC coefficient indicated significant differences compared with the control group (p<0.05), and GSH and MDA contents had extremely significant differences (p<0.01). These experimental results demonstrated that chlorpyrifos can increase the oxidative stress and DNA-protein crosslinks in mouse brain at a higher dose.