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环境科学学报 2012
The analyses of microbial community dynamics by T-RFLP during the bioaugmented removal of pyridine in SBR
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Abstract:
A bioaugmented reactor was developed through the introduction of Paracoccus sp. KT-5 into a sequencing batch reactor inoculated with activated sludge to treat pyridine-containing wastewater. The microbial community dynamics were analyzed by terminal restriction fragment length polymorphisms (T-RFLP) to evaluate the bioaugmentation effect. The results showed that the introduced strain KT-5 could accelerate the start-up of the SBR, in comparison with the non-bioaugmented one. However, the enhancing effect of bioaugmentation on pyridine degradation was not significant when the initial pyridine concentration increased to 195.6 mg·L-1. Moreover, the removal rate of pyridine was fluctuated when the initial pyridine concentration varied in the range of 293.4~586.8 mg·L-1. The analyses of T-RFLP showed that the introduced Paracoccus sp. KT-5 did not exist in the bioaugmented reactor when the initial pyridine concentration reached 978 mg·L-1, indicating that wash-out of strain KT-5 was probably responsible for the failure of bioaugmentation.