Bacterial biodiversity in the river in Northeast China during the freezing seasons: A case in Songhua River
我国东北典型河流冰封期细菌多样性的研究——以松花江为例

Five water samples were collected from different sites of the Songhua River in Northeast China during the freezing seasons. Community structure and species diversity of bacteria were investigated by constructing each bacterial 16S rRNA gene clone library and performing the phylogenetic analysis. The results showed that the five samples were lightly acidic, and were all contaminated to different degrees by polycyclic aromatic hydrocarbons (PAHs). The sample B was contaminated more seriously than the others. There were five phyla of bacteria detected from the all five samples, which were Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Cyanobacteria and Bacteroidetes. Many clones were affiliated with degrading bacteria, with the majority of those capable in degrading PAHs. The bacteria indicating eutrophication were detected in all samples, which implied eutrophication in the whole river, especially in the section where sample D and E were collected.