Isolation and identification of petroleum degrading bacteria and their remediation ability
高效石油降解菌的筛选鉴定及修复能力研究

In this paper, three strains of bacteria, WTS, Z3-P and H4-1, were isolated from petroleum-contaminated soils sampled from oil fields in Shandong Shengli, Xinjiang Karamay and Shaanxi Changqing. The ability of the strains to degrade diesel was examined, and after 10days the results showed that each strain was able to remove more than 60% of the total petroleum hydrocarbons, the WTS was proved that it had the most powerful ability, and the removal was 75%. When the strains were applied to real petroleum\|contaminated soils, the degradation half-life of petroleum was about 30 days, and reduced to less than one fourth of that under natural conditions. Physiological and biochemical analyses and 16S rRNA sequencing were conducted to identify the three strains. The results showed that WTS, H4-1 and Z3-P belong to Citrobacter sp., Alcaligenes xylosoxydans, and Bacillus sp., respectively.