Effects of the combined exposure of DEHP and OVA on lung function and pulmonary IL-17 expression of balb/c mice
DEHP与OVA联合染毒对小鼠肺功能和肺部IL-17表达的作用

To explore the effects and the related mechanism of di-(2-ehtylhexyl)phthalate (DEHP) on mice airway with and without ovalbumin (OVA) combined exposure, 24 male balb/c mice were randomly divided into four groups, with each group containing 6 mice: 1Untreated control (saline) group, 2DEHP exposure group, 3OVA exposure group and 4DEHP and OVA combined exposure group. The mouse asthma model was developed by OVA sensitization and challenge.The DEHP exposure group was given 10 mg·kg-1 DEHP daily by gavage for 54 consecutive days.The OVA exposure group and DEHP+OVA exposure group were given aerosol challenges in 1% OVA (30 min·d-1) once a day for a week from the 54th day to the 60th day to induce the asthma syndrome. On the 60th day, lung function testing, bronchoalveolar lavage fluid (BALF) collection and lung tissue interleukin-17A (IL-17A) measurement were undertaken. The results demonstrated that compared with DEHP exposure group, the DEHP+OVA exposure group increased IL-17A content significantly (p<0.05), reduced lung function (p<0.05), airway remodeling and pulmonary cells infiltration. It is suggested that the mechanism of DEHP induced asthma may be involved in IL-17 mediated effect.