Decolorization and degradation of Congo Red by Schizophyllum sp. F17 and catabolite analysis
裂褶菌F17对偶氮染料刚果红的脱色降解及其产物分析

Congo Red (CR) was decolorized and degraded by the main enzymes secreted by Schizophyllum sp. F17 which were investigated in a novel system established by our laboratory. Catabolite analysis showed that CR was decolorized and degraded effectively by Schizophyllum sp. F17. The decolorization percentage of CR reached 91.5% 48 h after mycelial pellet addition and the maximum activity of MnP, the main degradation enzyme, reached 96.1 U·L-1. Furthermore, from the UV-Vis spectrum of CR, the absorption peak at 495 nm vanished and new absorption peaks appeared in the ultraviolet region after 96 h and 192 h of decolorization. Benzidine is initially produced in the degradation process. It was isolated by HPLC and its identity was confirmed by FT-IR, mass spectrometry and NMR. The benzidine was itself degraded gradually over time.