Cloning of toxin detoxification-related liver genes in Taiwan snakehead (Channa maculata) and other aquatic animals
斑鳢等鱼贝类去毒相关基因的克隆

Liver genes that regulate detoxification are important in the metabolism of xenobiotics in various aquatic organisms. Cytochrome P450 1A (CYP1A) cDNAs of Taiwan snakehead (Channa maculata) and silver carp (Hypophthalmichthys molitrix), as well as heat shock protein 70 (HSP70) cDNAs of Taiwan snakehead, were obtained by RT-PCR methods. The cloned cDNA fragments were 908 bp, 902 bp, 684 bp in length, encoding 302, 300, 228 amino acids, respectively. Sequences of glutathione peroxidase (GPX) cDNAs were also cloned from the livers of Taiwan snakehead and Jinjiang oyster (Crassostrea ariakensis) using RT-PCR and RACE methods. Results obtained from this research provide a basis for future study of the structure, function and regulation of these genes, and will be used to produce safe aquatic food.