MOLECULAR SIZE DISTRIBUTION OF AQUATIC HUMIC SUBSTANCES 1 .A sequential gel filtration chromatography method
水生腐殖酸分子量分布研究——Ⅰ.串联凝胶色谱法测定水生腐殖酸分子量分布

A sequential gel filtration chromatography coupled with UV detector was applied for the characterization of molecular size distribution of aquatic humic substances.The working range of the system using Sephadex G-25 and G-100 covers almost the whole range of the molecular size distribution of aquatic humic substances.The system was calibrated using standard proteins and a discontinuous function can fit the calibration curve well.