BENZOQUINONE DNA ADDUCTS ANALYZED BY NUCLEASE P1 MEDIATED 32P-POSTLABELLING
32P后标记新法研究DNA-苯醌加合物的生成

With the super sensitivity, high reproducibility and accuracy, nuclease P1 mediated 32P-postlabelling version has been used successfully to analyze the DNA-BQ adducts formed from in vitro cultured cells, reaction of benzoquinone with calf thymus DNA and nucleoside monophposphates. It has been shown that the major one, which consists of more than 70% of the total radioactivities of DNA-BQ adducts detected, is from the deoxycytidine (dC) modified by benzoquinone while a minor one from deoxyguanosine (dG). The method is ca pable of detecting 1 adduct in 109-1010 DNA bases.