STUDY ON WASTEWATER FROM FERMENTATION OF MONOSODIUM GLUTAMATE BY THE HYBRID CELLS BETWEEN PROKARYOTE AND EUKARYOTE MICROORGANISM STRAINS WITH RHODOPSEUDOMONAS SPHMROIDES AND SACCHMOMCES CEREVISIAE
光合细菌与酵母跨界融合(杂合)子发酵味精废水研究

Keywords: Rhodopseudomonas sphaeroids,Saccharomyces cerevisiae,protoplast fusion,shaking reaction,monosodium glutamate wastewater
酿酒酵母,味精废水,废水处理,光合细菌

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Abstract:

光合细菌球形红假单胞菌（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ ｓｐｈａｅｒｏｉｄｅｓ）酿酒酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓ ｃｅｒｅｖｉｓｉａｅ）细胞分别经溶菌酶和蜗牛酶脱壁形成原生质体，在聚乙二醇（ＰＥＧ）和Ｃａ＾２＋诱导下实现了真核与原核细胞的超远缘杂交，经抗菌素培养基初步鉴定，融合产物能同时在含有链霉素和制霉菌素的培养基中生长，而双亲细胞只能在含有单一抗菌素的培养基中生长，经初筛与得获得高絮弟性

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