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OALib Journal期刊
ISSN: 2333-9721
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Degradation of purified terephthalic acid and expression of mnp gene for GEM Fhhh
遗传工程菌Fhhh降解精对苯二甲酸与mnp基因表达

Keywords: inter\|kingdom protoplast fusant,Phanerochaete chrysosporium,purified terephthalic acid,the specific degradation rate,relative factors,manganese peroxidase
跨界隔合子
,黄孢显毛平革菌,精对苯二甲酸,比降解率,相关因素,锰过氧化物酶,基因表达,Fhhh,废水处理,遗传工程菌,降解

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Abstract:

The levels of the specific degradation rate (SDR) for the genetically engineered microorganism (GEM) Fhhh from inter\|kingdom protoplast fusion and the parental strain \%Phanerochaete chrysosporium\% (PC) in degradation of purified terephthalic acid (PTA) were affected by (NH\-4)\-2C\-4H\-4O\-6,H\-2O\-2,Mn\+\{2+\},and pH value.Except pH value,the other 3 factors affecting the SDR levels had a same order for both strains.The pH value in the order was at the first site for Fhhh,and that for PC at the third site.At the optimal levels,the SDR value of Fhhh was 11\^11% higher than that of PC;the value of manganese peroxidase specific activity (MnP\|SA) of Fhhh was 15\^22% higher than that of PC.There was a positive relationship between SDR and MnP\|SA significantly.The optimal levels of 4 factors for SDR also were the optimal levels for mnp gene expression.The technique of the mnp gene expression control would be useful to treat PTA wastewater.

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