Studies on damage of membrane lipid peroxidation and deesterification caused by ozone treatment in Laminaria japonica
臭氧处理对海带膜脂过氧化和脱酯化伤害研究

Changes in photosynthesis rates,membrane relative permeability,H_2O_2 content,membrane lipid peroxidation and deesterification in Laminaria japonica under ozone treatment were studied by ecotoxicological and biochemical method.The results showed that the photosynthesis rates decreased with the ozone treatment intensity increment in Laminaria 901 and Laminaria No.1.Membrane relative permeability and H_2O_2 content increased,phospholipid content decreased and free fatty acid content promoted in microsomal membrane.These meant that membrane damage was seriously induced by ozone treatment and the increase in membrane damage was relatied to the accumulation of active oxygens which initiated deesterification.However,malondiadehyde(MDA) content in Laminria 901,not only in homogenate but also in microsomal membrane,did not show significant changes,while MDA content of microsomal membrane in Laminaria No.1 exhibited obvious changes.The results suggested that the membrane damage induced with ozone treatment was due to membrane lipid deesterification in Laminaria 901,and both membrane lipid deesterification and peroxidation in Laminarina No.1.