The applications of molecular biological tools to microbial ecosystem analyses in wastewater treatment process
分子生物学技术在污水处理微生物检测中的应用

The use of molecular biological tools to microorganism detections and community analyses in the biological wastewater treatment process had been discussed, and the major points were put on the more frequently used techniques such as polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH). The advantages and disadvantages of each technique were analyzed. Through the detections and analyses, the information of the amounts, the community structures and the activities of the microorganism in the system could be abtained, so these informations can be used to build enhanced ecosystem and to strengthen the efficiency of the existed system. It is believed that, the molecular biological tools based on the DNA sequences and the relevant structure genes have been successfully used in community analyses in the biological wastewater treatment process, and will surely help to get a deeper insight into and optimize the biological treatment process. Furthermore, it will promote the developments and applications of novel techniques, and play an even more important role in the process controlling of the biological wastewater treatments.