REDUCTIVE METABOLISM OF ENVIRONMENTAL CARCINOGEN 2-NITRO-FLUORENE BY RAT LIVER S-9 FRACTIONS
2-硝基芴在大鼠肝匀浆S-9组分作用下的还原代谢

Nitro polycyclic aromatic hydrocarbons such as 1-nitro-pyrene, 2-nitro-fluorene, 6-nitro-benzoa]pyrene and 4-nitro-biphenyl have been proved to be carcinogenic in mammalian assays. The mechanism of carcinogenesis is thought to be mediated by the metabolic reduction of these nitro compounds to reactive hydroxylamine intermediates by mammalian liver, lung,nasal tissue and intestinal microflo-ra. In this paper reductive metabolism of 2-nitro-fluorene by rat liver enzyme induced by Aroclor 1254 has been investigated under nitrogen atmosphere. The metabolites have been isolated by reversed phase HPLC and identified by comparison of their chromatogra-phic and UV spectral properties with the authentic compounds. The results show that the reductive metabolism of 2-nitro-fluorene yields mainly 2-amino-fluorene (a well-known carcinogen) plus three minor products, which remain unidentified because of insufficient quantities. The results of this study also confirm the complexity of the metabolic activation pathways.