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环境科学  2013 

Comparing Microbial Community of High Ammonia Wastewater and Municipal Sewage in a Partial Nitrification System
高氨氮废水与城市生活污水短程硝化系统菌群比较

Keywords: partial nitritation,biological nitrogen removal,16S rDNA clone library,phospholipid fatty acid (PLFA),quantitative PCR
短程硝化
,生物脱氮,16S,rDNA克隆文库,PLFA,定量PCR

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Abstract:

Nitritation is an important part of the biological nitrogen removal process, and the performance of the process was determined by the microbial community structure. To explore the microbial adaptability to different sewage, the microbial diversity and the amount of bacteria were investigated in a high ammonia wastewater treatment process and a sewage treatment process using the clone library of bacterial 16S rDNA, the phospholipid fatty acid method (PLFA) and the quantitative PCR. The clone library results showed that there was a significantly difference in bacterial community structure of these two processes, although the dominant bacteria belong to the Proteobacteria and Bacteroidete, there were more clusters in the sewage treatment process. The PLFAs results showed that the microbial diversity index and the evenness index of the high ammonium wastewater treatment process were significantly low. The quantitative PCR results showed that amounts of ammonia oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the high ammonium wastewater treatment process were higher than these in sewage treatment process. The copy number of AOB was higher than the copy number of NOB in the high ammonia wastewater treatment process by three orders magnitude. The copy number of AOB was higher than the copy number of NOB in sewage treatment process by two orders of magnitude.

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