Isolation, identification and degradation characteristics of three phenanthrene-degrading bacteria
三株菲降解细菌的分离、鉴定及降解特性的研究

Three bacterial strains capable of utilizing phenanthrene as sole carbon and energy sources were isolated from oil-contaminated soils. All of them were Gram-negative, aerobic, non-spore-forming, polar or side single flagellum rods and named strain ZX4, ZX6 and EVA17, respectively. They were considered to be belonged to Sphingomonas, strain ZX4 as S. paucimobilis, strain ZX6 as S. aromaticivorans and strain EVA17 was not identified to species, based on the results of physio-biochemical identification and the phylogenetical analyses of 16S rDNA sequence. The phylogenetic positions of the 3 strains were performed. The degradation rate of phenanthrene(1 000mg·L-1) by strain ZX4 was 98.74% within 14 d. Strain EVA17 had two types of metabolism pathway possibly showed by the degradation experiments of different substrates.