Comparison of Physicochemical Characterization of Shanghai Ambient Ultrafine Particles and Engineered Nano Particles and Their Cytotoxicity
上海大气超细颗粒物和工业纳米颗粒的表征及细胞毒性的比较研究

Ambient size-resolved particles in Shanghai atmosphere were collected at Shi Dong Kou sampling site during 2010 spring.Inductively coupled plasma atomic emission spectroscopy(ICP-AES) and field emission scanning electron microscopy(FESEM) were employed to investigate chemical elements and microscopic characterization of the ambient particles.Bioreactivity caused by the ambient particles and three kinds of engineered nano particles,NiO,ZnO and CeO2,was compared.Our results demonstrated that growth activity of A549 decreased and their intracellular ROS production intensity increased after the cell exposure to particle solution with different dosage(25,50,100 and 200 μg·mL-1).The lowest cell survival ratio was caused by soluble fraction of ambient fine particles,inhibition of cell viability were 13.31%,18.15%,20.43%,23.78% respectively.However,the most toxic components among the ultrafine ambient particles and engineered nano particles was NiO,inhibition of cell viability were 11.81%,15.12%,17.62%,19.44% respectively.From our results,we can conclude that the water-soluble fractions of the ambient fine particles are the most cytotoxic components among the measured particles.