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环境科学 2010
Effect of Long-term Fertilization on the Diversity of Nitrite Reductase Genes (nirK and nirS) in Paddy Soil
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Abstract:
In order to investigate the effects of long-term application of nitrogen fertilizer on soil denitrifying communities,the diversities of nir genes(nirK and nirS) were studied using molecular approaches in the long-term paddy field experiment(started in 1990) located in Taoyuan.Analysis of clone sequences indicated that the nirK fragments from paddy soil showed close similarity(90.7%) to the nirK sequences registered in GenBank database,but were not related to any known strain.Whereas,most of the nirS clones ...