Impact of PAHs on the Expression of PRDX in Earthworm (Eisenia fetida)
蚯蚓体内过氧化物还原蛋白PRDX基因对土壤PAHs污染胁迫的转录响应

A peroxiredoxin6 (PRDX6)-like gene segment has been found in up-regulated cDNA libraries of earthworm Eisenia fetida exposed to benzoa]pyrene(BaP). Analysis with basic local alignment search tool (BLAST) and phylogenetic analysis revealed that the gene segment contains a characteristic motif which was encoded by Cys, illustrating that this gene segment belongs to PRDX. To verify the response of PRDX in E. feitida exposed to poly aromatic hydrocarbons (PAHs), four-week pollution experiments were conducted following the methods recommended by the Organization for Economic Co-operation and Development (OECD). The results showed that the expression of PRDX was up-regulated in earthworm exposed to 1.0 mg·kg-1 Pyrene and BaP. It was indicated that PRDX in E. fetida was the potential molecular biomarkers of antioxidative stress caused by soil contamination.