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环境科学  2011 

Diversity Analysis of Desulfuration Bacterium from the Oxidation Ditch of City Sewage Treatment Plant with SO2 Gas
SO2诱导驯化城市污水处理厂氧化沟微生物的多样性分析

Keywords: desulfuration bacterium,16S rRNA sequencing,dioxide sulfurs,domestication,microbial diversity
脱硫菌
,16S,rRNA基因测序,二氧化硫,驯化,微生物多样性

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Abstract:

A group of removing SO2 bacterium was obtained from the oxidation ditch of city sewage treatment plant by inductive domestication over 6 d with low concentration SO2 gas, and they have an ability with biodegradation rate of 888 mg x (L x h)(-1) and a degradation efficiency of 85% during 1.5 h for SO2 dissolved in water with their synergy. The clone library and two phylogenetic trees of the removing SO2 bacterium communities were obtained based on 16S rRNA DNA comparison by DNA extraction of the sample and in situ polymerase chain reaction (PCR). The phylogenetic analysis showed that 8 dominant desulfuration bacterium occupy about 69% of all removing SO2 bacterium, and some of them have a kindred with discovered desulfuration bacterium but not homogeneity, and there are four belong to alpha-Proteobacteria, another four belong to beta-Proteobacteria in them. The gene information about 16S rRNA sequence of the dominant desulfuration bacteria and domestication method provide a basic of looking for or domesticating removing SO2 bacterium for development microbial desulfurization technology of contained SO2 tail gas.

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