Isolation of a Bacterial Strain Capable of Nitrobenzene-Degradation at Low Temperature and the Biodegradation Characteristics
低温硝基苯降解菌的筛选及降解特性研究

Seven strains that can degrade nitrobenzene at low temperature were isolated from the sediments of a nitrobenzene polluted river. One of the strains, NB1, can mineralize 20mg/L nitrobenzene completely under the temperature range from 2.5°C to 35°C with an optimum temperature of 25°C. At 5°C, the strain can grow and degrade 20mg/L nitrobenzene under pH 6-9. As long as the concentration of nitrobenzene was not higher than 100mg/L, it can be degraded by the strain successfully. It is identified as Pseudomonas putida according to its morphology, and biochemical properties, and 16S rDNA sequence analysis. The growth and nitrobenzene degradation character of the strain at different temperature, especially at low temperature, shows the potential for the bioremediation of nitrobenzene contaminated environment.