Isolation of Achromobacter xylosoxidans NS12 and Degradation of Nitrophenols
Achromobacter xylosoxidans NS12的分离和对硝基苯酚的降解

A nitrophenols-degrading bacterium, strain NS12, was isolated from a mangrove sediment by enrichment culture under aerobic conditions. Based on the analysis of 16S rDNA gene sequence the isolate was identified as Achromobacter xylosoxidans Strain NS12 was able to metabolize both O-nitrophenol (ONP) and P-nitrophenol (PNP) as the sole source of carbon, energy and nitrogen. However, this strain was not able to use 3-nitrophenol (MNP) as the only source of carbon energy and nitrogen for growth. The study demonstrated that when PNP and ONP occurred as a mixed substrate PNP degradation restrained the degradation of ONP and caused the major carbon source shift from ONP to PNP. Moreover, the results showed nitrophenols could be degraded by the indigenous bacteria in mangrove sediment.