Toxicity Evaluation of Sewage Treatment Plant Effluent of Chemical Industrial Park Along the Yangtze River on Rat Testicular Germ Cells in vitro
沿江化工园区污水处理厂出水对体外培养的大鼠睾丸细胞生殖毒性研究

By using rat testicular germ cells in vitro toxicity testing method based on original cells culture, the reproduction toxicity of sewage treatment plant effluent of Chemical Industrial Park along the Yangtze River was evaluated, through cells changes in morphologic, activity and viability parameters. The results showed that both of the effluents from new developed Chemical Industrial Park A and provincial Chemical Industrial Park B contain reproductive toxic substances. The toxicity of Park A has more significant undergone changes in cells activity of sertoli cells (p<0.01), spermatogenic cells (p<0.05) and leyding cells (p<0.05), lactate dehydrogenase activity (p<0.01) and testosterone secretion (p<0.01) than that of Park B. Sepermatogenic cells are more sensitive in indicating reproduction toxicity for testicular, compared with leyding cells and sertoli cells. This study demonstrated that, as an indispensable and complementary tool for water quality assessment, rat testicular germ cells in vitro toxicity testing based on original cells culture can be used to comprehensively evaluate the reproduction toxicity of sewage treatment plant effluent, and provide prompt and useful discharge quality information.