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环境科学 2009
16S rDNA Clone Library Analysis of Microbial Diversity Associated with the PSP-producing Dinoflagellate Alexandrium tamarense
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Abstract:
The composition of bacterial community in the Alexandrium tamarense culture was determined by analyzing the 16S rDNA clone libraries.16S rRNA gene was amplified by using the total DNA extracted from the A.tamarense culture at three growth stages as templates.34 different restriction fragment length polymorphism(RFLP) patterns of the clones were obtained from the three libraries.Clones representing each RFLP patterns were sequenced and phylogenetic analysis revealed that the Proteobacteria and the Bacteroide...
