Decoloration and Bioaugmentation on Azo Dye by Immobilized Genetically Engineered Strain
固定化工程菌对偶氮染料脱色及强化作用

Decoloration and bioaugmentation on Azo dye is investigated by using immobilized genetically engineered strain Escherichia coli JM109 (pGEX-AZR) on marcroporous foam carriers. The kinetics of the acid red GR decolorization by the immobilized E. coli JM109 (pGEX-AZR) accorded with Andrews model proved by our experiments, the kinetic parameters, , and , are found to be 49.2 mg?(g?h)-1 , 710.43 mg?L-1 and 681.62 mg?L-1 respectively. For continuous operating in the Anaerobic SBRs with 10% inoculation of Escherichia coli JM109 (pGEX-AZR) on marcroporous foam carriers for 32 days, both the tolerance to red GR concentration shocked and the colorific removal in the bioaugmented Anaerobic SBRs are higher than the control system, the acid red GR decoloration rate was reached 90%. Changes in microbial community have been detected by the RISA, in which the introduced immobilized GEM and preponderant mixed culture were subsisted steadily in sludge systems.