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环境科学 2007
Aerobic Denitrification of Nitrate Wastewater and Changes of Microbial Community Structure in a Bio-ceramic Reactor
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Abstract:
Biological treatment of nitrate wastewater with aerobic denitrifiers in a bio-ceramic reactor was investigated. And denaturing gradient gel electrophoresis (DGGE) method was used for analyzing the stability of microbial community structure. At the condition of the influent with hydraulic loading 0.75 m/h, gas/water ratio 5:1-10:1, temperature 15-23 degrees C, COD loading rate 1.92-5.98 kg/(m3 x d) and NO3- -N loading rate 0.60-1.34 kg/(m3 x d), high nitrate nitrogen removal of almost 100% and the maximum total nitrogen removal of up to 95.73% were achieved during stable operation stage. In addition, the nitrite concentration of effluent was lower. The PCR-DGGE profiles showed that shift of microbial diversity corresponded to the effect of nitrate removal and the preponderant populations for aerobic denitrification were steady during the test period.