Isolation and Identification of a PAHs-Degrading Strain Gordonia sp. He4 and Its Dynamics During Bioremediation of Phenanthrene Polluted Soil
降解多环芳烃的菌株Gordonia sp. He4的分离鉴定及其在菲污染土壤修复过程中的动态变化

A bacterial strain, He4, capable of degrading n-hexadecane and other polycyclic aromatic compounds was isolated from petroleum polluted soil. This strain was identified as Gordonia sp. He4 according to its morphology, physiological, biochemical properties and the analysis of its 16S rRNA gene sequence. Based on its 16S rRNA gene sequence, specific primers were designed and a competitor template was amplified by PCR. The dynamics of strain He4 in phenanthrene polluted soil was analyzed by colony forming unit (CFU) method and QC-PCR method. The results showed that partial of He4 become non-culturable and un-detectable by CFU method. But by using QC-PCR, the population density of strain He4 could be measured accurately.