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环境科学  2009 

Application of PCR-DGGE in Selective Activation of Stratal Bacteria
PCR-DGGE技术在内源细菌选择性激活过程中的应用

Keywords: stratal bacteria,selective activation,DGGE,PCR sequencing
内源细菌
,选择性激活,DGGE,PCR测序

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Abstract:

With the development of molecular biology,the research on microflora in the environment is accelerated and a new research method for oil reservoir microflora is provided.Based on the methods of PCR-DGGE,the change of the structure of dominant microbial population in selective activation of stratal bacteria was studied.During activation,the change of samples' DGGE bandings under different pressure conditions(1 MPa and 10 MPa) was analyzed,the PCR alignment analysis of bacteria was studied,the dominant bacter...

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