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环境科学  2006 

Biodiversity of Acidophiles in the Sediment at an Acid Mine Drainage Site
酸性矿山废水区域沉积物中嗜酸菌多样性研究

Keywords: acid mine drainage,sediment,acidophile,16S rDNA,DGGE
酸性矿山废水
,沉积物,嗜酸菌,16SrDNA,变性梯度凝胶电泳

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Abstract:

A sediment sample was collected at an acid mine drainage site in Anhui. The acidophiles composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis (DGGE). The results indicated that the acidophiles in the sediment were novel compared with the data in GenBank. The acidophiles affiliated with Acidobacteria, beta/gamma-Proteobacteria, delta-Proteobacteria, Nitrospira, Candidate Division TM7, low G + C Gram-positive. delta-Proteobacteria were major group in this acidic ecosystem. The research of aicdophiles community in sediment can help us develop high efficient method to treat acid mine drainage (AMD).

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