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环境科学  2006 

Diversity and Quantity of Different Microorganisms in Methanogenic Granular Sludge
高效厌氧产甲烷颗粒污泥中微生物多样性及定量化研究

Keywords: methanogenic granule sludge,microbial community structure,PCR-DGGE,FISH,RTQ-PCR
产甲烷颗粒污泥
,微生物群落结构,PCR-DGGE,FISH,RTQ-PCR

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Abstract:

PCR-DGGE,FISH and RTQ-PCR techniques were used to study the microbial community structure and quantity of different microorganisms in the methanogenic granule in different phases from a lab-scale anaerobic reactor.The results indicated that as the organic loading rate of the reactor increasing,the archaea community changed more significantly than bacterial community.Most bacteria were located in the out layer of granule,while most archaea were located in the inner layer.The quantity of archaea was a little less than bacteria,and the quantity of methanosaeta increased significantly.

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